Nanoparticle Tracking Analysis of Polymer Nanoparticles in Blood Plasma

A successful drug delivery system must overcome complex biological barriers. For particles injected into the blood, one of first and most critical barriers pertains to blood stability circulate through human body. To effectively design vehicles, interactions between as well aggregation behavior, be understood. This work presents a method analyze particle size in plasma using commercially available nanoparticle tracking analysis (NTA) system. As model system, fluorescently labeled polystyrene nanoparticles are incubated goat analyzed NTA. The found have protein corona that is larger than what has been observed by dynamic light scattering (DLS) diluted plasma. Particles decorated with PEG layer also large coronas undiluted Because NTA based on unique visualization method, multicomponent aggregates could quantified manner not feasible other techniques. PEGylation decrease from 1000 ± 200 for unmodified 30 1K PEGylated per 1 × 105 total particles.